Статья: Incorporation of 23456-2H5Phenylalanine 35-2H2Tyrosine and 24567-2H5Tryptophan into the Bacteriorhodopsin

Water-soluble products

of cellular content,

inorganic salts,

and other low-molecular-weight

compounds

Distilled H₂ O

RNase I,

125 mM NaCl, 20 mM MgCl,

4 mM Tris-HCl

Distilled H₂ O

Isolation of the biomass

Raw biomass
________ t

Osmotic shock

Culture liquid

4.3 M NaCl, and other

inorganic salts

and metabolites

50% ethanol

1.0.5%SDS-Na 2. Methanol

-5°C

-5°C

PM fraction

Decarotenoidation

±

Delipidation + BR precipitation

— Extract of carotenoids

_._ Residuals of cellular walls, lipids, and other high-molecular-weight compounds

Crystalline BR
t

Gel-permeation chromatography on Sephadex G-200