Курсовая работа: Vectors for moleculars cloning

7. Phage Lambda as a vector

7.1. Size Limitation for Packaging

7.2. Transfection of Recombinant Molecules

7.3. Biological Containment

8. Phage vectors

8.1.Replacement Vectors

8.2. Insertion Vectors

8.3.Storage of Lambda Stocks

Conclusion

Literature

Key words

Cosmids - an extrachromosomal circular DNA molecule that combines features of plasmids and phage; cloning limit - 35-50 kb.

DNA – a long chain polymer of deoxyribonucleotides. DNA constitutes the genetic material of most known organisms and organelles, and usually is in the form of a double helix, although some viral genomes consist of a single strand of DNA, and others of a single- or a double-stranded RNA.

Enzyme – a biological catalyst, usually a protein, that can speed up a chemical reaction by lowering it’s energy of activation without being used up in the reaction. Helicase – a type of enzyme that breaks hydrogen bonds between complementary base pairs of DNA, thereby causing the double strand to spit into separate single strands.

Molecular cloning – is process of creating an identical copy of DNA fragments. Phage - derivatives of bacteriophage lambda; linear DNA molecules, whose region can be replaced with foreign DNA without disrupting its life cycle. Plasmid - an extrachromosomal circular DNA molecule that autonomously replicates inside the bacterial cell.

Promoter - a specific DNA sequence that serves as a binding site for RNA polymerase near each gene.

Replicon – a block of DNA between two adjacent replication origins.

Vector – is an agent that can carry out a DNA fragment into a host cell.

Conditional shortenings

BAC – Bacterial Artificial Chromosome

cos – cohesice end site

DNA - deo xyribonucleic acid

Kb – Kilobases

Kbp – Kilobase pair

nt - necleotides

PCR – Polymarase chain reaction

pUc, pBluscript – phagemid vectors