Реферат: The effect of light intensity on the amount of chlorophyll in “Cicer arietinum”

Total

9

24

9

26

26

24

11

Table 3 (alternative) Frequency of length of 3-weeks-old plants depending on light intensity.

Light intensity, lux
Plant length (Class)	0,0	20,5	27,5	89,5	142,0	680,0	1220,0
0.0-10.0	0	0	0	19,23%	0	4,17%	0
10.1-20.0	0	0	11,10%	23,08%	0	4,17%	72,72%
20.1-30.0	0	0	0	50%	3,85%	41,62%	27,28%
30.1-40.0	0	37,50%	66,60%	7,69%	34,62%	37,52%	0
40.1-50.0	0	62,50%	22,30%	0	61,53%	12,52%	0
50.1-60.0	100%	0	0	0	0	0	0
Total	1	1	1	1	1	1	1

Calculation of the mean length of plants.

For light intensity equal to 20,50 lux:

The sum of lengths of all plants in this group is 45cm + 37cm + 39cm + 49cm + 46cm + 44cm + 45cm + 44cm + 42cm + 37cm + 40cm + 40cm + 39cm + 43cm + 42cm + 42cm + 36cm + 45cm + 38cm + 45cm + 46cm + 40cm + 35cm + 42cm + 43cm = 1044cm

Hence mean length is 1044cm : 25 plants = 41,76cm

Table 4.

Light intensity, lux	Mean wet biomass, g	Mean dry biomass, g	% of water	Mean length, cm	Mass of chl. In 1 g
0	0,273	0,041	84,98	41,89	0,0000
20,5	0,579	0,056	90,33	41,76	0,0496
27,5	0,332	0,033	90,06	36,33	0,1462
89,5	0,181	0,018	90,06	19,81	0,1769
142	0,511	0,047	90,80	41,33	0,0697
680	0,338	0,043	87,28	29,33	0,1557
1220	0,301	0,034	88,70	18,64	0,1939

Calculation of amount of chlorophyll in plants basing on the results of titration

H₂ SO₄ + C₅₆ O₅ N₄ Mg => C₅₆ O₅ N₄ H + MgSO₄

Concentration of H₂ SO₄ is 0,01 M

C – concentration

V – volume

n – quantity of substancy

m – mass

Mr – molar mass

For light intensity equal to 20,5 lux.

n = V (in dm³ ) ∙ C

2 ∙ 10^-3 ∙ 0,01 = 2 ∙ 10^-5

n = m / Mr => m = n ∙ Mr

m = 2 ∙ 10^-5 ∙ 832 = 1,664 ∙ 10^-2 grams