Статья: Метилотрофные бактерии - источники изотопно-меченных Н-2 и С-13 аминокислот

14. Егорова Т.А., Ерёмин С.В., Митснер Б.И., Звонкова Е.Н., Швец В.И. // Биотехнология. - N5. - 1993. - С. 30-35.

15. Egorova T.A., Eremin S.V., Mitsner B.I., Zvonkova E.N., Shvets V.I. // J. of Chromatography B. - 1995. - V. 665. - P. 53-62.

16. Daniely B. et al. // J. Org. mass spectrometry. - 1989. - 24. - P. 225-229.

О.V. MOSIN

Moscow State Academy of Fine Chemical Technology named after M.V. Lomonosov, 117571.

METHYLOTROPHIC BACTERIA - AS THE SOURCES OF ISOTOPICALLY LABELED ² H - AND ¹³ C-AMINO ACIDS.

A possibility of using the various strains of methylotrophic bacteria for the preparation of amino acids labeled with stable isotopes ² Н и ¹³ С, both secreted into culture medium and obtaining from protein hydrolysates is investigated. The data on adaptaition of L-phynylalanine producing strain of facultative methylotrophic bacteria Brevibacterium methylicum to growth media containing 2 v/v.% С² Н₃ О² Н and 98 v/v.% ² Н₂ О and biosynthesis of L-phenylalanine are presented. For L-leucine producing strain of obligate methylotrophic bacteria Methylobacillus flagellatum the cultivation was carried out on a medium containing the ordinary water and 1 v/v.% ¹³ С₁ Н₃ ОН. The levels of ² Н- and ¹³ С incorporation in amino acids were studied using the electron impact mass-spectrometry method of methyl esters of dansyl -and carbobenzoxychloride - derivatives of amino acids. The levels of ² Н- and ¹³ C incorporation into amino acids while growing of methylotrophic bacteria on media containing 2 v/v CH₃ OH and 98 v/v.% ² Н₂ O, and 1 v/v.% ¹³ CH₃ OH and 99 v/v. H₂ O were found to vary from 97,5% to 95%.